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Wow Beautiful , from korea

Hello Why do we quantify the concentration of the protein before putting it on the western blot? I tried searching this up and sources say to standardize how much protein we add to each well but if we standarize it wont it become that all of our bands will be equal and we cannot compare expression between bands? Thanks

ahhhh your content is sooooo so good, thank you so frkn much.

Thank you for this video! I needed these for the my class lab report <3

Worth subscribing ❤

Great lesson! I'd recommend some of the scientific calculators that exist online for folks to take advantage of for the sake of speed. EndMemo, Graphpad QuickCalcs, and WolframAlpha are a few that come to mind immediately.

I noticed that I never commented to any of the videos, however I would like you to know that your laboratory experience contribute me substantially as a potential MSc Molecular Biology graduate. Thank you for your efforts.

Hi, Do you know how can we remove paralogs using bioinformatics?

Is O-CAS just qualitative or is there a way to look at absorbance or something to get some numbers for siderophore production? BTW I've learned to never try to quickly cool a glass bottle after autoclaving bc same thing happened to one of my buffers where the bottom broke off and I got imidazole buffer all over my PI 😂😭 I'm sticking to good 'ol newtonian cooling on the bench

You've given me the inspiration I need to get back to studying! Thanks!

Do you know if EZvision will work for ssDNA? Currently trying to visualize an ssDNA synthesis and I'm not getting any signal in the ssDNA lanes.

You are a life saver. I've watched many of your videos are really appreciate you sharing your years of experience with noobs like me who are trying to get into plasmid cloning and recombinant protein expression! 🙏

Amazing content! :D

Thank you ❤

Hi... I have a doubt.. how to thaw Protein sample from -20 ? Any suggestions?

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this is great! i have a presentation on this in biochem and i cant express how much this help, also great visuals hahaha

Thank you ❤

This was so useful and well explained!

Why would you need/want a filler protein like BSA?

my favorite scientist on the internet, youre so good at explaining the really practical matters, so much thanks :D

Unit canceling calculation reminds me of Chemistry AP exam haha

Hi, I have a plasmid sample with the size of around 2.5kb and I am doing restriction digest on the sample where the RE is very near to each other, and will cut out around 6-10bp. Since I have not been getting results in my ligation procedure. I am suspecting if there could be anything wrong with my digestion. Therefore, I would like to verify if my digestion is actually successful or has failed. And to differentiate between my uncut and uncut plasmid samples, with only 6-10bp difference, I might want to try to run the samples with polyacrylamide gel. However, after trying 4% and 6% gel, the bands showing are at the same position, and here, I am not sure if the digestion failed or the difference still cannot be differentiated. Then, my senior suggested to use 20% gel and I did, but nothing showed up on my gel. I no longer know what to do now. If you can maybe help give some suggestions, I would greatly appreciate them. Thank you.

this is so good thank you

This helps! Thanks!

I often get a white residue/little ‘filaments’ when i spray my gloves with 70% ethanol. Have you had that happen? and if so, do you know why? I’m assuming it’s due to coating or residue on the glove itself, but I’ve been wondering if maybe it’s a sign that our di tap isn’t working properly

I have my entrance tests in 3 days and I wish I found your channel earlier. Love the content❤❤Thank you for the clear and concise explanations

When using the same syringe between different wells how can we avoid contamination? If it’s just used to pop the bubbles is that okay? Does contamination even matter for the Bradford assay? Apologies for so many questions, great video once again! 💖

Hello from iran! This is so cool! Thank you for sharing it on youtube!

guys this is the org chem tutor in practice

Resuspending pellets from a 1L culture is a pain still...even with vortexing+titruation...

ChimeraX is just as good as Pymol now. And ChimeraX is free.

This video is great (The diagrams, narration, show+tell) - it's more detailed and actionable than the videos and written guides provided by Addgene itself!

But doesn't 70% ethanol mean there are 7 parts ethanol and 3 parts water in a volume of 10 parts meaning if u do it that way u would and up adding too much water?

Such a pain in the A** for mixing in pcr tubes specially !

Hey! Great videos! I am a master student from Germany and following you. Does splicing or alternative splicing have a relationship with enzyme variation (like in CYP enzymes)? I know that it is about aminoacid changes in certain positions, but how about splicing? How it affects drug metabolism (biotransformation)? Why different isoforms give different response to same drug? Does it have a relationship with e.g. recombinant proteins produced in bacteria sometimes missing some sugar motifs and this proteins shows different activity and to compansate this, sometimes these motifs are added synthetically. Maybe this is a different way to think but I wanted find a connection between different topics

Hi, thanks very much for the video, it helps me understand a lot. I'm not very clear what do you mean at 3:05. WB only tells the presence of one type of protein, so do SDS and what at the same time?

hey , really greatful for what you're doing with us you have an amazing talent , actualy i'm a cancer researcher and I'm preparing my final Master's project , i want to ask u some questions so may i email u please thanks an advance

So cool!

This video is perfect! I'm learning how to grow bacteria to express proteins, and this demystified the options available; have each variant jotted down a notes document now.

Hey I have a question, so basically I want to look up the science behind things I want to look up. Like I want to know the stages of testosterone being increased . I don’t want to know the general answer like increasing testosterone is by sleep and muscles it says in google , I want to know the stages behind testosterone being increased.

Amazing video!

Great explanation! Loved it, thank you!

OMG WooooooooooooW🎉🎉🎉hope one day I can be like you 😢

Amazing, 15 mLs sounds like a lot! How many liters was your E. coli growth? What is the concentration of your undiluted stock? I'd like to compare it to my yields 😂

Been slowly discovering your videos and just wanted to say thank you so much for the content you make 😭🙏 it’s been a blessing in just a few days in aiding me in understanding the things and process in lab as a rising sophomore undergrad; my only regret is to not have found these videos sooner 🙇‍♂️

Is there any real prospect for someone with a mere bachelor's degree in bioengineering (somehow the syllabus barely contained any real wet lab skills aside from my interning/final year project) and not going for post-grad studies, and aiming to get into research, perhaps as a research assistant? I have heard that it is a dead-end job...

I thought I wanted to do something else with my life then when I really thought about it, there aren’t other careers that interest me enough to devote most of my waking hours to. So now I am 27 and considering STARTING grad school, feeling like a fool.

thank u internet mom

I really, really appreciate your videos and the resources you put in your descriptions. Thank you so much.